Review



selective h2s fluorescent dye wsp-1  (Cayman Chemical)


Bioz Verified Symbol Cayman Chemical is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Cayman Chemical selective h2s fluorescent dye wsp-1
    Selective H2s Fluorescent Dye Wsp 1, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wsp-1+dye/h+2+s+specific+fluorescent+probe+sf7+am/pm38857651-77-19-26
    Average 90 stars, based on 1 article reviews
    selective h2s fluorescent dye wsp-1 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Anti-Proliferative Properties of the Novel Hybrid Drug Met-ITC, Composed of the Native Drug Metformin with the Addition of an Isothiocyanate H 2 S Donor Moiety, in Different Cancer Cell Lines
    Article Snippet: After 24 h, the culture medium was replaced with 180 μL of a freshly prepared solution of the Washington State Probe-1 (WSP-1) dye (Cayman Chemical, Ann Arbor, MI, USA), dissolved in DMSO obtaining a stock solution of 2 mM and then diluted in buffer standard at the final concentration of 100 μM (buffer composition: KCl 2 mM, NaCl 120 mM, CaCl 2 ·2H 2 O 2 mM, HEPES 20 mM, glucose 5 mM and MgCl 2 ·6H 2 O 1 mM; pH 7.4).

    Article Title: The H 2 S-Donor Erucin Exhibits Protective Effects against Vascular Inflammation in Human Endothelial and Smooth Muscle Cells
    Article Snippet: After 24 h, the medium was replaced with a freshly prepared solution of WSP-1 (Washington State Probe-1, 3′-methoxy-3-oxo-3Hspiro[isobenzofuran-1,9′-xanthen]-6′-yl-2(pyridin-2-yldisulfanyl benzoate); Cayman Chemical, Ann Arbor, MI, USA), 100 μM.

    Article Title: Anti-Proliferative Properties of the Novel Hybrid Drug Met-ITC, Composed of the Native Drug Metformin with the Addition of an Isothiocyanate H 2 S Donor Moiety, in Different Cancer Cell Lines.
    Article Snippet: After 24 h, the culture medium was replaced with 180 μL of a freshly prepared solution of the Washington State Probe-1 (WSP-1) dye (Cayman Chemical, Ann Arbor, MI, USA), dissolved in DMSO obtaining a stock solution of 2 mM and then diluted in buffer standard at the final concentration of 100 μM (buffer composition: KCl 2 mM, NaCl 120 mM, CaCl2·2H2O 2 mM, HEPES 20 mM, glucose 5 mM and MgCl2·6H2O 1 mM; pH 7.4).



    Similar Products

    wsp 1 dye  (MedChemExpress)
    94
    MedChemExpress wsp 1 dye
    ( A ) Splenocytes from Pmel mice were stained with CTV dye and stimulated with gp100 antigen (1 μg/ml) + IL-2 (100 IU/ml) followed by staining <t>with</t> <t>WSP-1</t> dye to quantify H 2 S production using FACS ( n = 4 independent samples). ( B ) RT-PCR analysis performed on Pmel CD8 + T cells at various time points following activation to quantify mRNA levels of CBS ( n = 4). ( C and D ) Pmel T cells treated with 0.5 mM GYY4137 for 3 days. (C) FACS analysis for expression of CD62L and CD44 ( n = 4). (D) FACS analysis for expression of Tcf1/7 and Sca-1 ( n = 4). ( E and F ) WT and Cbs-KO splenocytes activated with anti-CD3 and anti-CD28 and expanded with addition of H 2 S to the Cbs-KO cells. (E) Analysis of intracellular H 2 S by FACS using WSP-1 dye and (F) FACS analysis of relative percentage of CM to EM cells ( n = 3). ( G and H ) Activated Pmel T cells transduced with lentiviral vector to overexpress CBS enzyme (Cbs td ) and on day 7 analyzed for (G) intracellular H 2 S production using WSP-1 dye and (H) frequency of CM (CD62L + CD44 + ) and Sca-1 expression ( n = 4). ( I to L ) B16-F10 tumors implanted in C57BL/6 mice and resected on day 14 to culture in vitro. Supernatant from the cultured tumor cells was extracted and transferred to cultures containing Pmel T cells and gp100 antigen with or without 0.5 mM GYY4137. T cells cultured under the conditions of the exhaustion assay were analyzed for expression of (J) CBS by FACS and (K) intracellular H 2 S using WSP-1 dye and quantified by FACS ( n = 4). (L) T cells from tumor supernatant exhaustion assay were also analyzed for the expression of CD62L and CD44 via FACS ( n = 4). All data shown represent the mean ± SEM and were analyzed by two-sided Student’s t test or one-way analysis of variance (ANOVA). ns, P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; ****P ≤ 0.0001.
    Wsp 1 Dye, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wsp-1+dye/WSP-1/pmc11566994-431-25-27
    Average 94 stars, based on 1 article reviews
    wsp 1 dye - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    dcfda dye  (MedChemExpress)
    94
    MedChemExpress dcfda dye
    ( A ) Splenocytes from Pmel mice were stained with CTV dye and stimulated with gp100 antigen (1 μg/ml) + IL-2 (100 IU/ml) followed by staining <t>with</t> <t>WSP-1</t> dye to quantify H 2 S production using FACS ( n = 4 independent samples). ( B ) RT-PCR analysis performed on Pmel CD8 + T cells at various time points following activation to quantify mRNA levels of CBS ( n = 4). ( C and D ) Pmel T cells treated with 0.5 mM GYY4137 for 3 days. (C) FACS analysis for expression of CD62L and CD44 ( n = 4). (D) FACS analysis for expression of Tcf1/7 and Sca-1 ( n = 4). ( E and F ) WT and Cbs-KO splenocytes activated with anti-CD3 and anti-CD28 and expanded with addition of H 2 S to the Cbs-KO cells. (E) Analysis of intracellular H 2 S by FACS using WSP-1 dye and (F) FACS analysis of relative percentage of CM to EM cells ( n = 3). ( G and H ) Activated Pmel T cells transduced with lentiviral vector to overexpress CBS enzyme (Cbs td ) and on day 7 analyzed for (G) intracellular H 2 S production using WSP-1 dye and (H) frequency of CM (CD62L + CD44 + ) and Sca-1 expression ( n = 4). ( I to L ) B16-F10 tumors implanted in C57BL/6 mice and resected on day 14 to culture in vitro. Supernatant from the cultured tumor cells was extracted and transferred to cultures containing Pmel T cells and gp100 antigen with or without 0.5 mM GYY4137. T cells cultured under the conditions of the exhaustion assay were analyzed for expression of (J) CBS by FACS and (K) intracellular H 2 S using WSP-1 dye and quantified by FACS ( n = 4). (L) T cells from tumor supernatant exhaustion assay were also analyzed for the expression of CD62L and CD44 via FACS ( n = 4). All data shown represent the mean ± SEM and were analyzed by two-sided Student’s t test or one-way analysis of variance (ANOVA). ns, P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; ****P ≤ 0.0001.
    Dcfda Dye, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wsp-1+dye/WSP-1/pmc11566994-431-19-27
    Average 94 stars, based on 1 article reviews
    dcfda dye - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    selective h2s fluorescent dye wsp-1  (Cayman Chemical)
    90
    Cayman Chemical selective h2s fluorescent dye wsp-1
    ( A ) Splenocytes from Pmel mice were stained with CTV dye and stimulated with gp100 antigen (1 μg/ml) + IL-2 (100 IU/ml) followed by staining <t>with</t> <t>WSP-1</t> dye to quantify H 2 S production using FACS ( n = 4 independent samples). ( B ) RT-PCR analysis performed on Pmel CD8 + T cells at various time points following activation to quantify mRNA levels of CBS ( n = 4). ( C and D ) Pmel T cells treated with 0.5 mM GYY4137 for 3 days. (C) FACS analysis for expression of CD62L and CD44 ( n = 4). (D) FACS analysis for expression of Tcf1/7 and Sca-1 ( n = 4). ( E and F ) WT and Cbs-KO splenocytes activated with anti-CD3 and anti-CD28 and expanded with addition of H 2 S to the Cbs-KO cells. (E) Analysis of intracellular H 2 S by FACS using WSP-1 dye and (F) FACS analysis of relative percentage of CM to EM cells ( n = 3). ( G and H ) Activated Pmel T cells transduced with lentiviral vector to overexpress CBS enzyme (Cbs td ) and on day 7 analyzed for (G) intracellular H 2 S production using WSP-1 dye and (H) frequency of CM (CD62L + CD44 + ) and Sca-1 expression ( n = 4). ( I to L ) B16-F10 tumors implanted in C57BL/6 mice and resected on day 14 to culture in vitro. Supernatant from the cultured tumor cells was extracted and transferred to cultures containing Pmel T cells and gp100 antigen with or without 0.5 mM GYY4137. T cells cultured under the conditions of the exhaustion assay were analyzed for expression of (J) CBS by FACS and (K) intracellular H 2 S using WSP-1 dye and quantified by FACS ( n = 4). (L) T cells from tumor supernatant exhaustion assay were also analyzed for the expression of CD62L and CD44 via FACS ( n = 4). All data shown represent the mean ± SEM and were analyzed by two-sided Student’s t test or one-way analysis of variance (ANOVA). ns, P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; ****P ≤ 0.0001.
    Selective H2s Fluorescent Dye Wsp 1, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wsp-1+dye/h+2+s+specific+fluorescent+probe+sf7+am/pm38857651-77-19-26
    Average 90 stars, based on 1 article reviews
    selective h2s fluorescent dye wsp-1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    wsp-1 dye  (Cayman Chemical)
    90
    Cayman Chemical wsp-1 dye
    ( A ) Splenocytes from Pmel mice were stained with CTV dye and stimulated with gp100 antigen (1 μg/ml) + IL-2 (100 IU/ml) followed by staining <t>with</t> <t>WSP-1</t> dye to quantify H 2 S production using FACS ( n = 4 independent samples). ( B ) RT-PCR analysis performed on Pmel CD8 + T cells at various time points following activation to quantify mRNA levels of CBS ( n = 4). ( C and D ) Pmel T cells treated with 0.5 mM GYY4137 for 3 days. (C) FACS analysis for expression of CD62L and CD44 ( n = 4). (D) FACS analysis for expression of Tcf1/7 and Sca-1 ( n = 4). ( E and F ) WT and Cbs-KO splenocytes activated with anti-CD3 and anti-CD28 and expanded with addition of H 2 S to the Cbs-KO cells. (E) Analysis of intracellular H 2 S by FACS using WSP-1 dye and (F) FACS analysis of relative percentage of CM to EM cells ( n = 3). ( G and H ) Activated Pmel T cells transduced with lentiviral vector to overexpress CBS enzyme (Cbs td ) and on day 7 analyzed for (G) intracellular H 2 S production using WSP-1 dye and (H) frequency of CM (CD62L + CD44 + ) and Sca-1 expression ( n = 4). ( I to L ) B16-F10 tumors implanted in C57BL/6 mice and resected on day 14 to culture in vitro. Supernatant from the cultured tumor cells was extracted and transferred to cultures containing Pmel T cells and gp100 antigen with or without 0.5 mM GYY4137. T cells cultured under the conditions of the exhaustion assay were analyzed for expression of (J) CBS by FACS and (K) intracellular H 2 S using WSP-1 dye and quantified by FACS ( n = 4). (L) T cells from tumor supernatant exhaustion assay were also analyzed for the expression of CD62L and CD44 via FACS ( n = 4). All data shown represent the mean ± SEM and were analyzed by two-sided Student’s t test or one-way analysis of variance (ANOVA). ns, P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; ****P ≤ 0.0001.
    Wsp 1 Dye, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wsp-1+dye/fluorescent+dye+wsp+1/pm38003321-427-18-23
    Average 90 stars, based on 1 article reviews
    wsp-1 dye - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    fluorescent dye wsp-1  (Cayman Chemical)
    90
    Cayman Chemical fluorescent dye wsp-1
    ( A ) Splenocytes from Pmel mice were stained with CTV dye and stimulated with gp100 antigen (1 μg/ml) + IL-2 (100 IU/ml) followed by staining <t>with</t> <t>WSP-1</t> dye to quantify H 2 S production using FACS ( n = 4 independent samples). ( B ) RT-PCR analysis performed on Pmel CD8 + T cells at various time points following activation to quantify mRNA levels of CBS ( n = 4). ( C and D ) Pmel T cells treated with 0.5 mM GYY4137 for 3 days. (C) FACS analysis for expression of CD62L and CD44 ( n = 4). (D) FACS analysis for expression of Tcf1/7 and Sca-1 ( n = 4). ( E and F ) WT and Cbs-KO splenocytes activated with anti-CD3 and anti-CD28 and expanded with addition of H 2 S to the Cbs-KO cells. (E) Analysis of intracellular H 2 S by FACS using WSP-1 dye and (F) FACS analysis of relative percentage of CM to EM cells ( n = 3). ( G and H ) Activated Pmel T cells transduced with lentiviral vector to overexpress CBS enzyme (Cbs td ) and on day 7 analyzed for (G) intracellular H 2 S production using WSP-1 dye and (H) frequency of CM (CD62L + CD44 + ) and Sca-1 expression ( n = 4). ( I to L ) B16-F10 tumors implanted in C57BL/6 mice and resected on day 14 to culture in vitro. Supernatant from the cultured tumor cells was extracted and transferred to cultures containing Pmel T cells and gp100 antigen with or without 0.5 mM GYY4137. T cells cultured under the conditions of the exhaustion assay were analyzed for expression of (J) CBS by FACS and (K) intracellular H 2 S using WSP-1 dye and quantified by FACS ( n = 4). (L) T cells from tumor supernatant exhaustion assay were also analyzed for the expression of CD62L and CD44 via FACS ( n = 4). All data shown represent the mean ± SEM and were analyzed by two-sided Student’s t test or one-way analysis of variance (ANOVA). ns, P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; ****P ≤ 0.0001.
    Fluorescent Dye Wsp 1, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wsp-1+dye/fluorescent+dye+wsp+1/pm37524208-60-49-57
    Average 90 stars, based on 1 article reviews
    fluorescent dye wsp-1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Splenocytes from Pmel mice were stained with CTV dye and stimulated with gp100 antigen (1 μg/ml) + IL-2 (100 IU/ml) followed by staining with WSP-1 dye to quantify H 2 S production using FACS ( n = 4 independent samples). ( B ) RT-PCR analysis performed on Pmel CD8 + T cells at various time points following activation to quantify mRNA levels of CBS ( n = 4). ( C and D ) Pmel T cells treated with 0.5 mM GYY4137 for 3 days. (C) FACS analysis for expression of CD62L and CD44 ( n = 4). (D) FACS analysis for expression of Tcf1/7 and Sca-1 ( n = 4). ( E and F ) WT and Cbs-KO splenocytes activated with anti-CD3 and anti-CD28 and expanded with addition of H 2 S to the Cbs-KO cells. (E) Analysis of intracellular H 2 S by FACS using WSP-1 dye and (F) FACS analysis of relative percentage of CM to EM cells ( n = 3). ( G and H ) Activated Pmel T cells transduced with lentiviral vector to overexpress CBS enzyme (Cbs td ) and on day 7 analyzed for (G) intracellular H 2 S production using WSP-1 dye and (H) frequency of CM (CD62L + CD44 + ) and Sca-1 expression ( n = 4). ( I to L ) B16-F10 tumors implanted in C57BL/6 mice and resected on day 14 to culture in vitro. Supernatant from the cultured tumor cells was extracted and transferred to cultures containing Pmel T cells and gp100 antigen with or without 0.5 mM GYY4137. T cells cultured under the conditions of the exhaustion assay were analyzed for expression of (J) CBS by FACS and (K) intracellular H 2 S using WSP-1 dye and quantified by FACS ( n = 4). (L) T cells from tumor supernatant exhaustion assay were also analyzed for the expression of CD62L and CD44 via FACS ( n = 4). All data shown represent the mean ± SEM and were analyzed by two-sided Student’s t test or one-way analysis of variance (ANOVA). ns, P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; ****P ≤ 0.0001.

    Journal: Science Advances

    Article Title: H 2 S-Prdx4 axis mitigates Golgi stress to bolster tumor-reactive T cell immunotherapeutic response

    doi: 10.1126/sciadv.adp1152

    Figure Lengend Snippet: ( A ) Splenocytes from Pmel mice were stained with CTV dye and stimulated with gp100 antigen (1 μg/ml) + IL-2 (100 IU/ml) followed by staining with WSP-1 dye to quantify H 2 S production using FACS ( n = 4 independent samples). ( B ) RT-PCR analysis performed on Pmel CD8 + T cells at various time points following activation to quantify mRNA levels of CBS ( n = 4). ( C and D ) Pmel T cells treated with 0.5 mM GYY4137 for 3 days. (C) FACS analysis for expression of CD62L and CD44 ( n = 4). (D) FACS analysis for expression of Tcf1/7 and Sca-1 ( n = 4). ( E and F ) WT and Cbs-KO splenocytes activated with anti-CD3 and anti-CD28 and expanded with addition of H 2 S to the Cbs-KO cells. (E) Analysis of intracellular H 2 S by FACS using WSP-1 dye and (F) FACS analysis of relative percentage of CM to EM cells ( n = 3). ( G and H ) Activated Pmel T cells transduced with lentiviral vector to overexpress CBS enzyme (Cbs td ) and on day 7 analyzed for (G) intracellular H 2 S production using WSP-1 dye and (H) frequency of CM (CD62L + CD44 + ) and Sca-1 expression ( n = 4). ( I to L ) B16-F10 tumors implanted in C57BL/6 mice and resected on day 14 to culture in vitro. Supernatant from the cultured tumor cells was extracted and transferred to cultures containing Pmel T cells and gp100 antigen with or without 0.5 mM GYY4137. T cells cultured under the conditions of the exhaustion assay were analyzed for expression of (J) CBS by FACS and (K) intracellular H 2 S using WSP-1 dye and quantified by FACS ( n = 4). (L) T cells from tumor supernatant exhaustion assay were also analyzed for the expression of CD62L and CD44 via FACS ( n = 4). All data shown represent the mean ± SEM and were analyzed by two-sided Student’s t test or one-way analysis of variance (ANOVA). ns, P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; ****P ≤ 0.0001.

    Article Snippet: In addition, MitoTracker Red (Cell Signaling Technology no. 9082), LIVE/DEAD Fixable Yellow Dead Cell Stain Kit (Invitrogen no. L34959), DCFDA dye (Abcam no. ab113851), and WSP-1 dye (MCE no. HY-124409) were used to evaluate mitochondrial mass, cell viability, cellular ROS, and H 2 S production, respectively, following the manufacturer’s protocol.

    Techniques: Staining, Reverse Transcription Polymerase Chain Reaction, Activation Assay, Expressing, Transduction, Plasmid Preparation, In Vitro, Cell Culture